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The effect of TGF-beta on the induction CD8 and NK1.1 expression in CTLL-2 cell line
- The effect of TGF-beta on the induction CD8 and NK1.1 expression in CTLL-2 cell line
- Cho, YJ; Lee, JS
- Ewha Authors
- 조영주; 이지수
- SCOPUS Author ID
- 조영주; 이지수
- Issue Date
- Journal Title
- JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY
- vol. 13, no. 4, pp. 259 - 265
- TGF-beta; CD8; NK cells; CTLL-2 cell lines
- HOGREFE & HUBER PUBLISHERS
- SCIE; SCOPUS
- Background: The presence of CD8(+) T-cells expressing NK cell associated markers (TNK cells) has been observed in several experimental models, which suggests that NK cells may belong to the T-cell lineage. We used the CTLL-2 cell line, which is NK1.1(+) CD3(-)TCR(+) CD4(-) CD8(-) cells in the presence of IL-2, to investigate whether these cells can be switched to CD8(+) or CD4(+) cells, like TNK cells, by the TGF-beta. Methods: CTLL-2 cells were cultured with TGF-beta or other cytokines and activators in the presence of IL-2. In order to see the surface and intracytoplasmic antigen expression in a single-cell level, simultaneous surface CD4, CD8, TCR with NK 1.1, and intracytoplasmic NK 1.1 staining was performed and three-color flow cytometric analysis was performed. Results: During routine passage, less than 5% of cells were CD8a(+), although 20-40% of cells expressed CD8a when treated with IL-2 + TGF-beta, whereas TPA + Calcium ionophore, IFN-gamma, and TNF-alpha cause no significant changes in the proportion of CD8(+) cells. Twenty percent of CTLL-2 cells expressed NK1.1 with IL-2 treatment, and this expression was also increased up to 65%-70% with IIL-2 + TNF-beta. Furthermore, most of the CD8 positive cells showed intracytoplasmic NK1.1. Conclusion: Our results indicated that these would be useful models to investigate CD8 precursor potentials in populations of CD4(-)CD8(-) (double negative) cells and the relationship of NK1.1. These results also support a role for TGF-beta in T-cell differentiation and the hypothesis that T-cells and NK cells may have the same ontogeny.
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