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Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice

Title
Peroxiredoxin II is essential for sustaining life span of erythrocytes in mice
Authors
Lee, THKim, SUYu, SLKim, SHPark, DSMoon, HBDho, SHKwon, KSKwon, HJHan, YHJeong, SKang, SWShin, HSLee, KKRhee, SGYu, DY
Ewha Authors
이서구강상원
SCOPUS Author ID
이서구scopusscopus; 강상원scopus
Issue Date
2003
Journal Title
BLOOD
ISSN
0006-4971JCR Link
Citation
vol. 101, no. 12, pp. 5033 - 5038
Publisher
AMER SOC HEMATOLOGY
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Peroxiredoxins (Prxs) are a family of antioxidant proteins that reduce peroxide levels by using reducing agents such as thioredoxin. These proteins were characterized to have a number of cellular functions, including cell proliferation and differentiation and protection of specific proteins from oxidative damage. However, the physiological roles of the peroxiredoxins have not been determined. To clarify the physiological relevance of this protein type, we generated a mouse model deficient in Prx II, which is abundantly expressed in all types of cells. The Prx II-/- mice were healthy in appearance and fertile. However, they had splenomegaly caused by the congestion of red pulp with hemosiderin accumulation. Heinz bodies were detected in their peripheral blood, and morphologically abnormal cells were elevated in the dense red blood cell (RBC) fractions, which contained markedly higher levels of reactive oxygen species (ROS). The Prx II-/- mice had significantly decreased hematocrit levels, but increased reticulocyte counts and erythropoletin levels, indicative of a compensatory action to maintain hematologic homeostasis in the mice. In addition, a labeling experiment with the thiol-modifying reagent biotinylated iodoacetamide (BIAM) in Prx II-/- mice revealed that a variety of RBC proteins were highly oxidized. Our results suggest that Prx II-/- mice have hemolytic anemia and that Prx II plays a major role in protecting RBCs from oxidative stress in mice. (C) 2003 by The American Society of Hematology.
DOI
10.1182/blood-2002-08-2548
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일반대학원 > 생명·약학부 > Journal papers
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