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Induced expression of a RhoA-specifiic guanine nucleotide exchange factor, p190RhoGEF, following CD40 stimulation and WEHI 231 B cell activation

Title
Induced expression of a RhoA-specifiic guanine nucleotide exchange factor, p190RhoGEF, following CD40 stimulation and WEHI 231 B cell activation
Authors
Lee, JRHa, YJKim, HJ
Ewha Authors
이종란
SCOPUS Author ID
이종란scopus
Issue Date
2003
Journal Title
JOURNAL OF IMMUNOLOGY
ISSN
0022-1767JCR Link
Citation
vol. 170, no. 1, pp. 19 - 23
Publisher
AMER ASSOC IMMUNOLOGISTS
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Stimulation of the B cell surface receptor CD40 induces transcriptional activation and protein expression. To determine which proteins are required for the CD40-mediated B cell activation, we performed a two-dimensional gel electrophoresis of the WEHI 231 B cell lysates. We report in this study the identification of one protein in which the expression was remarkably induced following CD40 stimulation. It was the p190 Rho guanine nucleotide exchange factor (GEF), p190RhoGEF, a recently identified GEF that is specific for RhoA, Overexpression of either p190RboGEF or hboA (Q63L), a constitutively active form of RboA, mimics the effects of CD40 stimulation, such as changes in cellular structure and NF-kappaB activation. These p190RhoGEF overexpression effects are abrogated when coexpressed with a dominant negative form of RboA (T19N), We also provide evidence for the CD40-mediated cellular changes that are abrogated in cells that are overexpressed with the dominant negative form of either p190RhoGEF (Y1003A) or RboA (T19N).
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자연과학대학 > 생명과학전공 > Journal papers
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