View : 13 Download: 3

Bactericidal activity in whole blood as a potential surrogate marker of immunity after vaccination against tuberculosis

Title
Bactericidal activity in whole blood as a potential surrogate marker of immunity after vaccination against tuberculosis
Authors
Cheon, SHKampmann, BHise, AGPhillips, MSong, HYLanden, KLi, QLarkin, REllner, JJSilver, RFHoft, DFWallis, RS
Ewha Authors
전선희
SCOPUS Author ID
전선희scopus
Issue Date
2002
Journal Title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN
1071-412XJCR Link
Citation
vol. 9, no. 4, pp. 901 - 907
Publisher
AMER SOC MICROBIOLOGY
Indexed
SCIE WOS
Abstract
The development of new tuberculosis (TB) vaccines will require the identification of correlates of human protection. This study examined the balance between immunity and virulence in a whole blood infection model in which intracellular mycobacterial survival was measured using BACTEC. In the blood of tuberculin-negative donors, counts of Mycobacterium tuberculosis H37Ra organisms fell by 0.14 log(10) CFU during 96 h of whole blood culture, whereas counts of Mycobacterium bovis BCG, M. tuberculosis H(37)Rv, and a clinical TB isolate's organisms increased by 0.13, 0.43, and 1.04 log(10) CFU, respectively (P < 0.001), consistent with their relative virulence. Inhibition of tumor necrosis factor alpha by the addition of methylprednisolone or pentoxifylline or removal of CD4(+) or CD8(+) T cells by magnetic beads had deleterious effects on immune control of intracellular growth only in the blood of tuberculin-positive donors. Repeated vaccination of eight tuberculin-negative volunteers with M. bovis BCG resulted in a 0.3 log (50%) reduction in BCG CFU counts in the model compared to baseline values (P < 0.05). Three of the volunteers responded only after the second vaccination. These experiments indicate that whole blood culture may be used to measure immunity to M. tuberculosis and that further studies of repeated BCG vaccination are warranted.
DOI
10.1128/CDLI.9.4.901-907.2002
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
Bactericidal activity in whole blood as a potential surrogate marker of immunity after vaccination against tuberculosis.pdf(163.02 kB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE