Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이윤실 | * |
dc.date.accessioned | 2016-08-27T02:08:41Z | - |
dc.date.available | 2016-08-27T02:08:41Z | - |
dc.date.issued | 2002 | * |
dc.identifier.issn | 1044-9523 | * |
dc.identifier.other | OAK-1073 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215507 | - |
dc.description.abstract | Protein kinase C (PKC) has been widely implicated in regulation of cell growth/cell cycle progression and apoptosis. However, the role of PKCS in radiosensitivity and cell cycle regulation remains unclear. Overexpression of PKCdelta increased Ca2+- independent PKC activity without altering other PKC isoforms (PKCalpha, -beta1, -epsilon, and -zeta), and extracellular regulated protein kinase (ERK) 1/2 activity was also increased in PKCdelta-specific manner. A clonogenic survival assay showed that PKCdelta-overexpressed cells had more radiosensitivity and pronounced induction of apoptosis than control cells. Flow cytometric analysis revealed that PKCdelta made the cells escape from radiation-induced G(2)-M arrest. Moreover, p53 and p21(Waf) induction by radiation were higher in PKCdelta-overexpressed cells than control cells, and PKCdelta-mediated apoptosis was reduced, when radiation-induced ERK1/2 activity was inhibited by PD98059. Furthermore, PKCdelta antisense and rottlerin, PKC inhibitor-abrogated PKCS-mediated radiosensitivity and reduced ERK1/2 activity to the control vector level. These results demonstrated that PKCS overexpression enhanced radiation-induced apoptosis and radiosensitivity via ERK1/2 activation, thereby abolishing the radiation-induced G(2)-M arrest and finally apoptosis. | * |
dc.language | English | * |
dc.publisher | AMER ASSOC CANCER RESEARCH | * |
dc.title | Protein kinase C delta overexpression enhances radiation sensitivity via extracellular regulated protein kinase 1/2 activation, abolishing the radiation-induced G(2)-M arrest | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 13 | * |
dc.relation.index | SCIE | * |
dc.relation.startpage | 237 | * |
dc.relation.lastpage | 246 | * |
dc.relation.journaltitle | CELL GROWTH & DIFFERENTIATION | * |
dc.identifier.wosid | WOS:000176195500004 | * |
dc.author.google | Lee, YJ | * |
dc.author.google | Soh, JW | * |
dc.author.google | Dean, NM | * |
dc.author.google | Cho, CK | * |
dc.author.google | Kim, TH | * |
dc.author.google | Lee, SJ | * |
dc.author.google | Lee, YS | * |
dc.contributor.scopusid | 이윤실(17137192000) | * |
dc.date.modifydate | 20240130115944 | * |