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Molecular identification of vaginal Lactobacillus spp. isolated from Korean women

Title
Molecular identification of vaginal Lactobacillus spp. isolated from Korean women
Authors
Chang, CEPavlova, SITao, LKim, EKKim, SCYun, HSSo, JS
Ewha Authors
김승철
SCOPUS Author ID
김승철scopus
Issue Date
2002
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
1017-7825JCR Link
Citation
vol. 12, no. 2, pp. 312 - 317
Keywords
vaginal microfloraLactobacillus identificationPCR-RFLP16S rDNAantimicrobial activity
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS
Abstract
Indigenous lactobacilli were isolated from vaginas of Korean women for possible use in ecological treatment of bacterial vaginosis. Vaginal swab samples were obtained from a gynecological clinic and streaked on Rogosa SL agar plates to select the most predominant lactobacilli in each sample. The preliminary identification of the isolates as lactobacilli was based on microscopic observation of Gram-positive rod-shaped cell morphology. The initial characterization was performed on 108 isolates in terms of their cell surface hydrophobicity (CSH), antimicrobial activity, and hydrogen peroxide (H2O2) production capability, and 10 isolates were then selected for further molecular identification. For a rapid procedure to identify lactobacilli, polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses of the 16S rRNA genes were applied. The 10 selected lactobacilli and 9 different reference strains of Lactobacillus spp. were characterized by PCR-RFLP where the amplified 16S rDNA was digested with 7 different restriction endonucleases prior to analysis. DNA sequencing of the 16S rRNA gene of one particular isolate, KLB 46, that had been identified as L. crispatus by the PCR-RFLP analysis, further confirmed its identity as L. crispatus.
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의학전문대학원 > 의학과 > Journal papers
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