Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 한인옥 | - |
dc.date.accessioned | 2016-08-27T02:08:38Z | - |
dc.date.available | 2016-08-27T02:08:38Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.other | OAK-977 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215479 | - |
dc.description.abstract | HA95, a nuclear protein homologous to AKAP95, has been identified in immune precipitates of the Epstein-Barr virus (EBV) coactivating nuclear protein EBNA-LP from EBV-transformed lymphoblastoid cells (LCLs). We now find that HA95 and EBNA-LP are highly associated in LCLs and in B-lymphoma cells where EBNA-LP is expressed by gene transfer. Binding was also evident in yeast two-hybrid assays. HA95 binds to the EBNA-LP repeat domain that is the principal coactivator of transcription. EBNA-LP localizes with HA95 and causes HA95 to partially relocalize with EBNA-LP in promyelocytic leukemia nuclear bodies. Protein kinase A catalytic subunit alpha (PKAcsalpha) is significantly associated with HA95 in the presence or absence of EBNA-LP. Although EBNA-LP is not a PKA substrate, HA95 or PKcsalpha expression in B lymphoblasts specifically down-regulates the strong coactivating effects of EBNA-LP. The inhibitory effects of PKAcsalpha are reversed by coexpression of protein kinase inhibitor. PKAcsa also inhibits EBNA-LP coactivation with the EBNA-2 acidic domain fused to the Ga14 DNA binding domain. Furthermore, EBNA-LP- and EBNA-2-induced expression of the EBV oncogene, LMP1, is down-regulated by PKAcsalpha or HA95 expression in EBV-infected lymphoblasts. These experiments indicate that HA95 and EBNA-LP localize PKAcsalpha at nuclear sites where it can affect transcription from specific promoters. The role of HA95 as a scaffold for transcriptional regulation is discussed. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Protein kinase A associates with HA95 and affects transcriptional coactivation by Epstein-Barr virus nuclear proteins | - |
dc.type | Article | - |
dc.relation.issue | 7 | - |
dc.relation.volume | 22 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 2136 | - |
dc.relation.lastpage | 2146 | - |
dc.relation.journaltitle | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.identifier.doi | 10.1128/MCB.22.7.2136-2146.2002 | - |
dc.identifier.wosid | WOS:000174407500017 | - |
dc.author.google | Han, I | - |
dc.author.google | Xue, Y | - |
dc.author.google | Harada, S | - |
dc.author.google | Orstavik, S | - |
dc.author.google | Skalhegg, B | - |
dc.author.google | Kieff, E | - |
dc.contributor.scopusid | 한인옥(57197545599) | - |
dc.date.modifydate | 20211210152151 | - |