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dc.contributor.author유충규-
dc.date.accessioned2016-08-27T02:08:38Z-
dc.date.available2016-08-27T02:08:38Z-
dc.date.issued2000-
dc.identifier.issn1078-0297-
dc.identifier.otherOAK-971-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/215476-
dc.description.abstractVarious factors most likely to influence the plasma protein binding of IQO4, a new isoquinolinedione derivative, to 4% human serum albumin (HSA) were evaluated using an equilibrium dialysis technique at an initial IQO4 concentration of 5 mug/ml. It took approximately 12 h incubation to reach an equilibrium between 4% HSA and isotonic Sorensen phosphate buffer at pH 7.4 containing 3% dextran ('the buffer') using a Spectra/Por 2 membrane (molecular weight cut-off, 12000-14000) in a water-bath shaker kept at 37degreesC and at a rate of 50 oscillations per min. IQO4 was stable both in 4% HSA and in 'the buffer' for up to 24 h incubation at 37degreesC. The binding of IQO4 was constant (89.9 +/- 1.40%, mean +/- standard deviation) at IQO4 concentrations ranging from 1 to 100 mug/ml. However, the extent of binding was dependent on HSA concentrations. The values were 32.5, 62.0, 79.1, 84.9, 90.9, 91.2, and 91.7% at HSA concentrations of 0.5, 1, 2, 3, 4, 5, and 6%, respectively; on incubation temperature, 96.7, 93.8, and 91.0% when incubated at 4, 22, and 37degreesC, respectively; and on the buffer pHs, 84.4, 87.2, 88.2, 90.9, and 92.3% for the buffer pHs of 5.8, 6.4, 7, 7.4, and 8, respectively. The free fraction of IQO4 increased with the addition of sulfisoxazole (0-300 mug/ml), and salicylic acid (03 00 mug/ml). The protein binding of IQO4 was independent of the quantity of alpha-1-acid glycoprotein (up to 0.32%), chloride ion (up to 0.546%) and heparin (up to 40 units/ml).-
dc.languageEnglish-
dc.publisherP J D PUBLICATIONS LTD-
dc.titleFactors influencing the protein binding of IQO4, a new isoquinolinedione derivative-
dc.typeArticle-
dc.relation.issue3-4-
dc.relation.volume108-
dc.relation.indexSCIE-
dc.relation.startpage167-
dc.relation.lastpage177-
dc.relation.journaltitleRESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY-
dc.identifier.wosidWOS:000174280000003-
dc.author.googleMoon, YJ-
dc.author.googleKim, SH-
dc.author.googleRyu, CK-
dc.author.googleLee, MG-
dc.contributor.scopusid유충규(15846918400)-
dc.date.modifydate20180104081001-
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약학대학 > 약학과 > Journal papers
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