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Requirement for the L-type Ca2+ channel alpha(1D) subunit in postnatal pancreatic beta cell generation

Title
Requirement for the L-type Ca2+ channel alpha(1D) subunit in postnatal pancreatic beta cell generation
Authors
Namkung, YSkrypnyk, NJeong, MJLee, TLee, MSKim, HLChin, HSuh, PGKim, SSShin, HS
Ewha Authors
김형래
SCOPUS Author ID
김형래scopus
Issue Date
2001
Journal Title
JOURNAL OF CLINICAL INVESTIGATION
ISSN
0021-9738JCR Link
Citation
vol. 108, no. 7, pp. 1015 - 1022
Publisher
AMER SOC CLINICAL INVESTIGATION INC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Pancreatic 5 cells are the source of insulin, which directly lowers blood glucose levels in the body. Our analyses Of alpha (1D) gene-knockout (alpha (1D-/-)) mice show that the L-type calcium channel, alpha (1D), is required for proper beta cell generation in the postnatal pancreas. Knockout mice were characteristically slightly smaller than their littermates and exhibited hypoinsulinemia and glucose intolerance. However, isolated a(1D)(-/-) islets persisted in glucose sensing and insulin secretion, with compensatory overexpression of another L-type channel gene, alpha (1C). Histologically, newborn a(1D)(-/-) mice had an equivalent number of islets to wild-type mice. in contrast, adult alpha (-/-)(1D) mice showed a decrease in the number and size of islets, compared with littermate wild-type mice due to a decrease in beta cell generation. TUNEL staining showed that there was no increase in cell. death in alpha (-/-)(1D) islets, and a 5-bromo-2 ' deoxyuridine-labeling (BrdU-labeling) assay illustrated significant reduction in the proliferation rate of beta cells in alpha (-/-)(1D) islets.
DOI
10.1172/JCI13310
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의학전문대학원 > 의학과 > Journal papers
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