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Inhibitory effect of fangchinoline on excitatory amino acids-induced neurotoxicity in cultured rat cerebellar granule cells
- Inhibitory effect of fangchinoline on excitatory amino acids-induced neurotoxicity in cultured rat cerebellar granule cells
- Kim, SD; Oh, SK; Kim, HS; Seong, YH
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- vol. 24, no. 2, pp. 164 - +
- fangchinoline; excitatory amino acids; neurotoxicity; cerebellar granule cells
- PHARMACEUTICAL SOCIETY KOREA
- SCIE; SCOPUS; KCI
- Glutamate receptors-mediated excitotoxicity is believed to play a role in the pathophysiology of neurodegenerative diseases. The present study was performed to evaluate the inhibitory effect of fangchinoline, a bis-benzylisoquinoline alkaloid, which has a characteristic as a Ca2+ channel blocker, on excitatory amino acids (EAAs)-induced neurotoxicity in cultured rat cerebellar granule neuron. Fangchinoline (1 and 5 muM) inhibited glutamate (1 mM), N-methyl-D-aspartate (NMDA; 1 mM) and kainate (100 muM]-induced neuronal cell death which was measured by trypan blue exclusion test. Fangchinoline (1 and 5 muM) inhibited glutamate release into medium induced by NMDA (1 mM) and kainate (100 muM), which was measured by HPLC. And fangchinoline (5 muM) inhibited glutamate [1 mM)-induced elevation of intracellular calcium concentration. These results suggest that inhibition of Ca2+ influx by fangchinoline may contribute to the beneficial effects on neurodegenerative effect of glutamate in pathophysiological conditions.
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