Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍영숙 | * |
dc.contributor.author | 박혜영 | * |
dc.date.accessioned | 2016-08-27T02:08:31Z | - |
dc.date.available | 2016-08-27T02:08:31Z | - |
dc.date.issued | 2000 | * |
dc.identifier.issn | 1226-3613 | * |
dc.identifier.other | OAK-596 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215397 | - |
dc.description.abstract | Fas transduces apoptotic signals upon cross-linking with the Fas ligand (FasL), which is experimentally replaced by agonistic anti-fas monoclonal antibodies (mAb). Of eight human malignant hematopoietic cell lines (HL-60, KG-1, THP-1, K562, U937, Jurkat, IM-9, RPMI-8226) examined by flow cytometric analysis, all, except K562, were found to be positive for surface Fas antigen. However, despite surface Fas expression, the agonistic anti-Fas mAb (7C11) induced apoptosis in only three of seven Fas-expressing cell lines (KG-1,Jurkat and IM-9). This Fas-resistance did not correlated with high levels of mRNA either for DcR3, a decoy receptor for FasL, or for FAP-1, a Fas-associated phosphatase that can block the apoptotic function of Fas. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis did not show consistent differences in the expression of Bcl-2 and Bax between Fas-sensitive and Fas-resistant cell lines examined. These findings indicated that the presence or absence of mRNA expression of DcR3, FAP-1, Bcl-2 and Bax did not always correlate with relative sensitivity to Fas-mediated apoptosis. Treatment of cells with cycloheximide converted the phenotype of resistant cell lines from Fas-resistant to Fas-sensitive, and enhanced the sensitivity of Fas-sensitive cell lines. These results suggest that the Fas-resistance is dependent on the presence of labile proteins that determine resistance to Fas-mediated apoptosis and the apoptotic machinery is already in place in Fas-resistant cell lines. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | * |
dc.subject | apoptosis | * |
dc.subject | fas | * |
dc.subject | DcR3 | * |
dc.subject | FAP-1 | * |
dc.subject | Bcl-2 | * |
dc.subject | bax | * |
dc.subject | cycloheximide | * |
dc.title | Fas-mediated apoptosis and expression of related genes in human malignant hematopoietic cells | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 32 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 246 | * |
dc.relation.lastpage | 254 | * |
dc.relation.journaltitle | EXPERIMENTAL AND MOLECULAR MEDICINE | * |
dc.identifier.wosid | WOS:000166183400013 | * |
dc.identifier.scopusid | 2-s2.0-0034739829 | * |
dc.author.google | Kim, KM | * |
dc.author.google | Lee, K | * |
dc.author.google | Hong, YS | * |
dc.author.google | Park, HY | * |
dc.contributor.scopusid | 홍영숙(57218968598) | * |
dc.contributor.scopusid | 박혜영(7601567979) | * |
dc.date.modifydate | 20240301081003 | * |