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Responsiveness of the state of O-linked N-acetylglucosamine modification of nuclear pore protein p62 to the extracellular glucose concentration

Title
Responsiveness of the state of O-linked N-acetylglucosamine modification of nuclear pore protein p62 to the extracellular glucose concentration
Authors
Han, IOh, ESKudlow, JE
Ewha Authors
오억수
SCOPUS Author ID
오억수scopus
Issue Date
2000
Journal Title
BIOCHEMICAL JOURNAL
ISSN
0264-6021JCR Link
Citation
vol. 350, pp. 109 - 114
Keywords
N-acetylglucosamineO-glycosylationtranscription
Publisher
PORTLAND PRESS
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
O-linked N-acetylglucosamine (O-GlcNAc) modification has been described in many proteins, including nuclear pore glycoproteins. In the present study we investigated the effect of extracellular glucose on the level of modification of nuclear pore protein p62 by O-GlcNAc. We found that exposure of cells to a high concentration of glucose caused an increased level of modification of p62 with O-GlcNAc, whereas the modification of other proteins did not change detectably. The increased O-GlcNAc modification of p62 in response to glucose required the metabolism of glucose to glucosamine. The exposure of the cells to glucosamine itself also caused increased O-GlcNAc modification, whereas mannosamine and galactosamine did not. Furthermore, changes in glucose concentrations within the physiological range induced the O-GlcNAc modification in p62 in rat aortic smooth-muscle cells, indicating that this modification of p62 might occur in an intact animal. These results imply that the ambient glucose concentration has an impact on the level of O-GlcNAc modification of proteins such as p62, and that functional changes in some of these proteins might ensue.
DOI
10.1042/0264-6021:3500109
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자연과학대학 > 생명과학전공 > Journal papers
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