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Molecular cloning of clathrin assembly protein gene (rCALM) and its differential expression to AP180 in rat brain

Title
Molecular cloning of clathrin assembly protein gene (rCALM) and its differential expression to AP180 in rat brain
Authors
Kim, HLLee, SC
Ewha Authors
김형래
SCOPUS Author ID
김형래scopus
Issue Date
1999
Journal Title
EXPERIMENTAL AND MOLECULAR MEDICINE
ISSN
1226-3613JCR Link
Citation
vol. 31, no. 4, pp. 191 - 196
Keywords
clathrin-coated vesicleAP180CALMgene cloningexpression
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Binding of clathrin assembly protein to clathrin triskelia induces their assembly into clathrin-coated vesicle (CCV) in neurons. The clathrin assembly protein gene (rCALM) was cloned from rat brain cDNA library. rCALM deduced 69 kD molecule has overall 73% amino acid homology compared with that of AP180 protein. The N-terminal domain, where amino acid sequences are very similar with AP180, harbours binding sites for clathrin and inositides, as well as possible phosphorylation sites, but the proline rich C-terminal domain is different from that of AP180. The mRNA expression of rCALM and AP180 by in situ hybridization histochemistry revealed that the rCALM mRNA was more intensely expressed than that of AP180, and the distribution patterns were different from each other. These results suggest that the rCALM mediates the assembly of clathrin in neural and supporting cells of brain, and regulates the clathrin coated-vesicle formation through phosphorylation and inositide metabolism.
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의학전문대학원 > 의학과 > Journal papers
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