View : 14 Download: 0

Changes of [H-3]muscimol binding and GABA(A) receptor beta 2-subunit mRNA level by tolerance to and withdrawal from pentobarbital in rats

Title
Changes of [H-3]muscimol binding and GABA(A) receptor beta 2-subunit mRNA level by tolerance to and withdrawal from pentobarbital in rats
Authors
Oh, SHo, IK
Ewha Authors
오세관
SCOPUS Author ID
오세관scopus
Issue Date
1999
Journal Title
NEUROCHEMICAL RESEARCH
ISSN
0364-3190JCR Link
Citation
vol. 24, no. 12, pp. 1603 - 1609
Keywords
pentobarbitalmuscimol bindingGABA(A) receptor beta 2-subunitin situ hybridization
Publisher
KLUWER ACADEMIC/PLENUM PUBL
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Effects of continuous pentobarbital administration on binding characteristics of [H-3]muscimol were examined by autoradiography, and levels of GABA(A) receptor beta 2-subunit mRNA were investigated by in situ hybridization histochemistry in the rat brain. In order to eliminate the induction of hepatic metabolism by systemic administration of pentobarbital, an i.c.v. infusion model of tolerance to and withdrawal from pentobarbital was used. An experimental model of barbiturate tolerance and withdrawal was developed using i.c.v. infusion of pentobarbital (300 mu g/10 mu l/hr for 7 days) by osmotic minipumps and abrupt withdrawal from pentobarbital. The levels of [H-3]muscimol binding were elevated in cingulate of frontal cortex (46%) and granule layer of cerebellum (32%) of rats 24-hr after withdrawal from pentobarbital, while it was only elevated in cingulate (58%) of tolerant rats. The GABA(A) receptor beta 2-subunit mRNA was increased in the withdrawal rats only: in the cortex (9-14%), hippocampus (15-21%), inferior colliculus (21%), and granule layer of cerebellum (24%). These results show the involvement of GABA(A) receptor and its beta 2-subunit up-regulations in pentobarbital withdrawal rats, and suggest that the levels of [H-3]muscimol binding and GABA(A) receptor beta 2-subunit mRNA are altered in a region-specific manner during pentobarbital withdrawal.
DOI
10.1023/A:1021168519387
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE