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Genomic structure of the regulatory region of the voltage-gated calcium channel alpha 1D

Title
Genomic structure of the regulatory region of the voltage-gated calcium channel alpha 1D
Authors
Kim, HLChang, YJLee, SMHong, YS
Ewha Authors
홍영숙김형래
SCOPUS Author ID
홍영숙scopusscopus; 김형래scopus
Issue Date
1998
Journal Title
EXPERIMENTAL AND MOLECULAR MEDICINE
ISSN
1226-3613JCR Link
Citation
vol. 30, no. 4, pp. 246 - 251
Keywords
calcium channelgene expressionregulationmolecular cloning
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
In excitable and endocrine organs, calcium influxes through the L-type voltage-gated calcium channel (VGCC) which is composed of four (alpha 1, alpha 2, beta, and gamma) subunits. Temporal and spatial expression of calcium channel activity is regulated by the transcription of alpha 1 subunit. To elucidate the genomic organization of the VGCC alpha 1D subunit gene, a genomic clone was isolated from the human genomic library and its sequence was analyzed. A 12 kb genomic clone contained the 5'-flanking regulatory region and first two exons was selected and the initiation site for alpha 1D mRNA synthesis was examined by primer extension analysis. The major initiation site was found at the -523 NT position in the translation initiation site. The TATA box could not be found above the transcription initiation site. The CAT vector construct containing the 2.5 kb upstream region had high CAT activity on transfection to NG108-15 and PC12 cells, which confers the neuronal expression of the alpha 1D gene.
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의학전문대학원 > 의학과 > Journal papers
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