The effect of cytokine mediators on prostaglandin inhibition by human decidual cells

Abstract

OBJECTIVE: The purpose of this study was to determine the inhibitory effects of anti-interleukin-1 beta and transforming growth factor-beta(2) on the production of interleukin-1 beta and prostaglandin E-2 by human decidual cells. STUDY DESIGN: Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control, lipopolysaccharide plus anti-interleukin-1 beta, and lipopolysaccharide plus transforming growth factor-beta(2), respectively. Interleukin-1 beta and prostaglandin E-2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay. RESULTS: At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1 beta, anti-interleukin-1 beta decreased the lipopolysaccharide-induced prostaglandin E-2 production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P<.05) and also decreased the lipopolysaccharide-induced interleukin-1 beta production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P<.05). In the medium containing lipopolysaccharide plus transforming growth factor-beta(2), transforming growth factor-beta(2) also decreased the lipopolysaccharide-induced prostaglandin E-2 production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P<.05) but increased the lipopolysaccharide-induced interleukin-1 beta production significantly at the concentration of 1 ng/mL (P<.05). CONCLUSIONS: Lipopolysaccharide-induced prostaglandin E-2 production by human decidual cells in vitro may be prevented by anti-interleukin-1 beta and transforming growth factor-beta(2), and interleukin-1 beta production may be prevented by anti-interleukin-1 beta.