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The effect of cytokine mediators on prostaglandin inhibition by human decidual cells

Title
The effect of cytokine mediators on prostaglandin inhibition by human decidual cells
Authors
Kim, YJAhn, JJWoo, BH
Ewha Authors
안정자우복희김영주
SCOPUS Author ID
안정자scopus; 우복희scopusscopus; 김영주scopus
Issue Date
1998
Journal Title
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
ISSN
0002-9378JCR Link
Citation
vol. 179, no. 1, pp. 146 - 149
Keywords
preterm laborcytokinedecidual cellprostaglandin
Publisher
MOSBY-YEAR BOOK INC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
OBJECTIVE: The purpose of this study was to determine the inhibitory effects of anti-interleukin-1 beta and transforming growth factor-beta(2) on the production of interleukin-1 beta and prostaglandin E-2 by human decidual cells. STUDY DESIGN: Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control, lipopolysaccharide plus anti-interleukin-1 beta, and lipopolysaccharide plus transforming growth factor-beta(2), respectively. Interleukin-1 beta and prostaglandin E-2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay. RESULTS: At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1 beta, anti-interleukin-1 beta decreased the lipopolysaccharide-induced prostaglandin E-2 production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P<.05) and also decreased the lipopolysaccharide-induced interleukin-1 beta production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P<.05). In the medium containing lipopolysaccharide plus transforming growth factor-beta(2), transforming growth factor-beta(2) also decreased the lipopolysaccharide-induced prostaglandin E-2 production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P<.05) but increased the lipopolysaccharide-induced interleukin-1 beta production significantly at the concentration of 1 ng/mL (P<.05). CONCLUSIONS: Lipopolysaccharide-induced prostaglandin E-2 production by human decidual cells in vitro may be prevented by anti-interleukin-1 beta and transforming growth factor-beta(2), and interleukin-1 beta production may be prevented by anti-interleukin-1 beta.
DOI
10.1016/S0002-9378(98)70265-0
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의학전문대학원 > 의학과 > Journal papers
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