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감초 분획의 진통효과에 관한 연구

감초 분획의 진통효과에 관한 연구
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Studies on the analgesic effects of fractions of Glycyrrhizae Radix
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대학원 약학과
이화여자대학교 대학원
감초의 메탄올엑기스에서 진통작용을 확인하고 그 효과가 어느 분획에서 기인하는 가를 추적하고자 하였다. 감초 메탄올엑기스를 Hexane분획과 Ethyl acetate분획으로 분리 추출한 후, Ethyl acetate엑기스는 TLC를 이용하여 Rf치가 감초의 주성분인 Glycyrrhizin보다 높은 것을, 높은 순서대로 Rf치 가 유사한 것을 묶어 Column Chromatography로 분리하여 분획 Ⅰ~Ⅶ을 얻었다. 메탄올엑기스, Hexane엑기스, Ethyl acetate 엑기스의 진통작용실험 방법은 초산 Writhing법과 Randall-Selitto paw pressure법을 선택하여 실시하였다. 분획 Ⅰ~Ⅶ 및 Glycyrrhizin은 초산 Writhing법에 의해 진통실험을 하였다. 메탄올엑기스, Hexane엑기스, Ethyl acetate 엑기스는 용량 증가에 따라 진통효과도 증가됨을 보였으며, Hexane엑기스가 Ethyl acetate엑기스보다 적은 용량에서 진통효과가 큼을 보였다. 분획 Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅵ도 용량증가에 따라 진통효과가 증가됨을 보였다. Glycyrrhizin은 진통효과가 없었다.;This investigation aimed to study the analgesic effects of the extracts separated from Glycyrrhizae Radix. The methanol extracts of Glycyrrhizae Radix was fractionated into two parts; 1) hexane soluble fraction 2) ethyl acetate soluble fraction. The quantities of the hexane extract were much less than the ethyl acetate extract, therefore the ethyl acetate fraction was separated into eight parts by column chromatography. Seven fractions (Fraction Ⅰ-Ⅶ) which showed higher Rf value than glycyrrhizin were tested. Each of these seven fractions showed different Rf value on the silica gel TLC. The analgesic effects of methanol extract, hexane extract, ethyl acetate extract were determined by acetic acid writhing method and Randall-Selitto paw pressure method but those of seven fractions were determined only by acetic acid writhing method. The analgesic effects of all of the extracts were compared with those of acetyl salicylic acid. All of the extracts were administrated orally. The acetic acid writhing method was carried out in mice and the Randall-Selitto method was carried out in rats. The analgesic effects of the methanol extract, hexane extract, ethyl acetate extract were increased in dose-dependent manner. Their effects were statistically significant. In the mice treated with glycyrrhizin, the increase of writhing inhibition was statistically nonsignificant. Fraction Ⅰ-Ⅶ except fraction Ⅴ inhibited writhing syndrome in dose-dependent manner.
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