식이 섭취량의 제한과 회복으로 인한 골격근육내 성분변화

Abstract

본 연구는 성장이 왕성한 시기인 이유 직후에 식이섭취량을 감소시켜 체중증가를 제한시켰다가 회복시킬 때 그 회복과정을 보는데 목적이 있었다. 평균체중이 52.6±0.9g인 Sprague-Dawley종 젖 떨어진 수컷 흰 쥐 56마리를 총 8군으로 7마리씩 나누어서, 3주간 77% starch-15% caseintlr이로 섭취량을 조절하여서 체중증가를, 무제한, 0%로, 30%로 제한하였다가 그 후 17일 동안 동일식이를 무제한 섭취시켜서 회복시켰다. 전 실험기간동안 각 군마다 식이섭취량과 체중을 측정하였고, 실험동물들이 같은 몸무게에 도달했을 때와 같은 나이에 도달했을 때를 각각 비교하여 골격근육 - Anterior Tibialis, Extensor Digitorum Logus, Soleus, Plantaris, Gastrocnemius의 무게와 근육내 단백질, 수분함유율, Sodium, Potassium, Magnesium, Phosphorus, Carcass 단백질을 분석한다. 그 결과를 요약하면 다음과 같다. 1. 식이섭취량은 체중증가를 제한했던 3주동안 일주당 표준군이 103.9±6.5g을 섭취했으며, 30% 체중증가군은 53.9±0.7g, 0% 체중증ㄱ군은 43.4±0.5g으로 섭취량을 제한시켜서 체중증가량을 조절하였다. 그 이후는 무제한으로 식이를 섭취시켜서 회복시켰다. 2. 체중증가량은 식이섭취량의 임의조절로 체중증가를 제한했던 3주동안, 표준군이 106.8±12.57g 체중증가를 한 반면, 30% 체중증가군은 18.9±3.3g, 0% 체중증가군은 3.6±2.1g만이 증가되었다. 회복시켜서 나이 66일이 되었을 때에도 표준군이 여전히 다른 군보다 높은 체중을 나타내었다. 3. 근육의 젖은 무게와 Carcass 단백질은 식이섭취의 제한에 의해서 표준군, 30% 체중증가군, 0% 체중증가군의 순서로 높았으며, 회복시켜서 나이 66이이 되었을 때도 여전히 표준군이 체중증가를 제한 했었던 군보다 높았다. 4. 수분함유율은 식이섭취의 제한에 의해서 표준군이 체중증가를 제한했었던 군들보다 낮은 경향이었으나, 회복시켜서 나이 66일이 되었을 때는 각 군끼리 비슷해졌다. 5. 전해질의 농도는 그 종류에 따라서 식이섭취의 제한의 결과가 달랐다. Sodium과 Magnesium은 체중증가의 조절에 의해서 표준군이 체중증가를 제한했었던 군들보다 낮은 경향을 보였으나, 그 반면 Potassium과 Phosphorus는 표준군이 오히려 높은 경향을 나타내었다. 그러나 일단 회복시켜서 나이 66일이 되었을 때는 각 군들간에 비슷한 경향을 나타내었다. 6. 성장 초기의 식이섭취량 제한수준에 따라서 동일체중에 도달하는 시기는 다르지만, 일단 동일체중이되면 근육내 전해질의 농도는 같아지는 경향이었다.;This study was performed in order to investigate the changes of electrolytes composition in skeletal muscles by food restriction and rehabilitation. Fifty six male weanling rats of the Sprague - Dawley strain weighing 52.6±0.9g were fed with 77% starch - l5% casein diet by ad libitum for four days to get them adapted. Then they were divided into eight groups. For three weeks, the body weight gain of rats was controlled in three different ranges. The first range : Control group, which were fed by ad libitum. The second range : The Second group, no body weight gain. The third range : The Third group 30% body weight gain After the period of food restriction, the rats tore recovered by being fed by ad libitum for seventeen days. Doring the experimental period, the amount of food intake and body weight were measured. And the experimental groups were compared when they reached at the same age and at the same body weight. Anterior Tibialis, extensor Digitorum Longus, Soleus, Plantaris, Gastrocnemius were used as analytic items of skeletal muscle. Wet, weight of muscle, muscle protein, water content were measured from each of five skeletal muscles. Sodium, Potassium, Magnesium content were measured from Anterior Tibialis, Extensor Digitorum Longus, Soleus and Plantaris. Phosphorus was measured only from Gastrocnemius. The whole carcass protein except the skeletal muscles was also measured. The results were summarized as following : 1. During the period of food restriction, food intake was 311.7±19.5g for the control group, 130.2±1.5g for the second group and 161.7±2.1g for the third group. After that period, they were recovered by being fed by ad libitum, 2. During the period of food restriction, body weight gain was 106.8±12.57g for the control group, 3.6±2.1g for the second group and 18.9±3.3g for the third group. Comparing the body weight when they reached at the age 66 days, the control group showed higher value than the other groups. 3. In wet weight of muscles and whole carcass protein, the control group showed the highest value, next the third and the second group by food restriction. After their recovery, the control group showed still higher value than the other groups. 4. In the concentration of electrolytes of skeletal muscles Sodium ana Magnesium tend to increase and Potassium and Phosphorus tend to decrease by food restriction. But after their recovery, there was no significant difference between the groups. 5. By the degree of food restriction, there was time difference in reaching at the same body weight. But when they reached at the same body weight, there was no significant difference in the value of electrolytes concentration.