DL-1-Aminoethylphosphonic acid의 生物學的 機能 硏究

Abstract

Since 1959 B-aminoethylphosphonic acid was discovered in the living organism, the biosysthesis and biological functions of aminophosphonic acids have been extensively studied. The author designed and carried, out this study for 14 weeks to find out the metabolic function of Ethylaminophosphonic acid ( AEP )and its utilization in the living body. Sixty rats, thirty males and thirty females aged 40±5 days were divided into two parts, one for Alanine supplemented as control group and the other for AEP as experimental group to compare metabolic pathway of ordinary amino acid with that of AEP.. Both alanine and AEP groups were divided into two subgroups according to the level of supplements, 0.1 ％ and 0.2 ％ of the diet. The major components of the diet in this study was composed of 20 ％ Casein, 72 ％ sugar, 4 ％ fat, 4 ％ salt mixture and all kind of vitamins in adequate amount. For comparison of biological values between experimental and control groups in terms of body weight, urinary nitrogen, creatinine excretion and final body weight, there were no ststistically significant differences in these respects. This meant AEP could be utilized in the body as much as alanine could. Urinary phosphorus excretion was determined by developing the blue color to read on the Spec□ronic 20 . Statistically insignificance in tho urinary phosphor□ excretion between experimental and control groups was observed in spite of the supplementation of phosphorus of AEP for experimental group in the diet. The level of blood phosphorus was higher in. experimental group than that in control group, this result supported above result. In the analysis of fat and nitrogen contents in the liver , AEP group showed slightly higher than control group in both respects. But it was noteworthy 0.2 ％ AEP group in both sex were higher than 0.1 ％ AEP in liver fat content. Histological examinations of gaptrointestinal tract, liver, lung, spleen, heart, adrenal glands, kidney, and sex organs showed no changes in all groups included in this study. The group supplemented higher level of diet by alanine 0.2 ％ and AEP 0,2 ％ stayed on less body weight gain and lower liver weight. This result could be interpreted that amino acid imbalnaced condition was arose in the body.