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Growth factor and vitamin D loaded PLGA nanoparticles on anodized titanium implant enhances osteoblast-like cell differentiation

Growth factor and vitamin D loaded PLGA nanoparticles on anodized titanium implant enhances osteoblast-like cell differentiation
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치아이식용 임플란트에서 전기분사법을 이용한 성장인자와 비타민 D 함유 나노입자 코팅을 통한 표면 개질 방법
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대학원 생명·약학부약학전공
이화여자대학교 대학원
Osseointegration is an essential event to determine the success of dental implantation. Surface modification is an important approach for improving osseointegration in addition to reducing implant treatment time. Biochemical design is known as one of the effective approach because controlled and rapid healing would be possible. Basic FGF (fibroblast growth factor) and vitamin D were used by reason that they have biological activity on osteoblasts. Poly(lactide-co-glycolide) (PLGA) was used as a drug carrier and we coated titanium discs with electrospray technique. To determine the effect of growth factor and vitamin D on osteoblasts, we conducted the experiment with 4 groups - bare, bFGF loaded, vitamin D loaded and compound of two drugs loaded group. The release profile of loaded vitamin D was measured in vitro and characteristics of coated surface were observed by scanning electron microscopy (SEM). Human osteoblast-like cells (HOS) were used for examining bioactivity of two drugs. And CCK-8 (cell counting kit-8) assay and ALPase (alkaline phosphatase) test was conducted. The uniform PLGA nanoparticles were obtained through the electrospray coating technique. Vitamin D in the particle matrix was released for 10 days in vitro. FGF and vitamin D enhanced the differentiation activity of HOS cells. And the two drugs interacted with each other so they had synergic effect on osteoblasts. In conclusion, FGF and vitamin D are the potential drugs for improving the osseointegration of dental implant and the electrospray method enables to carry the drugs in the stable form.;치아이식용 임플란트의 표면 개질을 통한 골유착(osseointegration) 향상을 도모하기 위한 목적으로 전기분사(electrospray)방법을 이용하여 산화피막 처리된 타이타늄 임플란트의 표면에 조골세포의 증식을 촉진하는 성장인자인 bFGF와 분화인자인 비타민 D를 코팅하였다. 이들 약물은 고분자 나노입자에 함유된 상태로 임플란트 표면에 고르게 코팅되어 체내 이식 후 약물이 서방출할 수 있도록 고안되었다. 함유된 약물의 용출실험과 조골세포를 이용한 초기 부착실험, 증식능과 분화능에 관련된 실험을 통하여 제조된 임플란트가 생체친화성을 증가시켰음을 확인하였다. 이를 통해 전기분사방법을 이용한 생리활성물질의 코팅방법이 매우 효과적이며, 성장인자와 비타민 D 또한 치주용 임플란트의 표면 개질에 적합한 물질임을 입증하였다.
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