Full metadata record
DC Field | Value | Language |
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dc.contributor.advisor | 이경림 | - |
dc.contributor.author | Zhou Jialan | - |
dc.creator | Zhou Jialan | - |
dc.date.accessioned | 2016-08-26T11:08:16Z | - |
dc.date.available | 2016-08-26T11:08:16Z | - |
dc.date.issued | 2009 | - |
dc.identifier.other | OAK-000000051289 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/201936 | - |
dc.identifier.uri | http://dcollection.ewha.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000051289 | - |
dc.description.abstract | Lead exposure is a well-known cause of hypertension in humans and experimental animals. Although different considerations have been raised to explain the pathogenesis of lead-induced hypertension, several studies have suggested that the primary involvement of the increased production of reactive oxygen species (ROS) was observed in lead-exposed animals. Increased level of ROS can be generated by the upregulation of COX-2 and catalase. To identify whether TCTP is involved in the mechanism of lead-induced hypertension, I treated lead on rat aortic smooth muscle cells, A7r5. The expression of TCTP, COX-2, and catalase was increased by the lead treatment, and its expression level was decreased by TCTP siRNA transfection. In summary, lead caused the overexpression of TCTP, COX-2 and catalase which might be involved in the generation of ROS in A7r5 cells, suggesting that TCTP is most likely involved in the pathogenesis of lead-induced hypertension.;사람을 비롯한 동물 실험 모델에서 납(lead)이 고혈압 유발인자임을 밝혔다. 납으로 야기된 고혈압의 병태 생리학적 기전을 설명하는 여러 시도 중에 활성 산소 종(ROS)의 발생이 가장 유력한 고혈압 발생 원인일 수 있다고 납(lead)중독 동물모델 연구에서 보고되었다. 활성 산소 종(ROS)은 COX-2, catalase의 증가로 발생한다. TCTP가 납(lead)으로 야기된 고혈압 발생 기작에 관여하는 지를 증명하기 위해, 나는 A7r5 세포에 납을 처리하였고 TCTP가 과발현 됨을 확인하였다. 납에 의해 TCTP가 과발현 되었을 때 COX-2, catalase의 발현도 증가하였고, TCTP를 siRNA로 knockdown 시켰을 때 COX-2, catalase의 발현도 감소되는 것을 확인하였다. 요약하여, 납이 A7r5 세포에서 활성 산소 종(ROS)을 발생 시킬 수 있는 TCTP, COX-2, calatase를 과발현 시켰으므로, 이는 TCTP가 납(lead)으로 야기된 고혈압의 병태생리학적 기전에 관여될 수 있음을 시사한다. | - |
dc.description.tableofcontents | Ⅰ. Introduction = 1 Ⅱ. Experimental procedures = 11 A. Cell culture = 11 B. Lead treatments = 11 C. Western blotting analysis = 12 1. Antibodies = 12 2. Sample preparation = 13 3. Western blotting = 13 D. Transfection = 14 1. Preparation of TCTP siRNA = 14 2. Transfection protocol = 15 E. Statistics = 15 Ⅲ. Results = 18 A. Effect of lead on TCTP expression in A7r5 cells = 18 B. Downregulation of TCTP by TCTP siRNA transfection in lead-treated A7r5 cells = 21 C. Upregulation of COX-2 and catalase in lead-treated A7r5 cells and their downregulation by TCTP siRNA transfection = 23 Ⅳ. Discussion = 26 Ⅴ. References = 30 논문초록 = 40 | - |
dc.format | application/pdf | - |
dc.format.extent | 789360 bytes | - |
dc.language | eng | - |
dc.publisher | 이화여자대학교 대학원 | - |
dc.title | Overexpression of translationally controlled tumor protein as a potential mechanism for lead-induced hypertension | - |
dc.type | Master's Thesis | - |
dc.creator.othername | Zhou Jialan | - |
dc.format.page | ⅳ, 43 p. | - |
dc.identifier.thesisdegree | Master | - |
dc.identifier.major | 대학원 생명·약학부약학전공 | - |
dc.date.awarded | 2009. 2 | - |