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Studies on Isolation, Identification, and Biological Activities of Antifungal Antibiotics produced by Streptomyces willmorei EMP22, against Cryptococcus laurentii

Title
Studies on Isolation, Identification, and Biological Activities of Antifungal Antibiotics produced by Streptomyces willmorei EMP22, against Cryptococcus laurentii
Authors
은소영
Issue Date
1997
Department/Major
대학원 생물과학과
Keywords
StreptomycesEMP22Cryptococcus laurentiiIsolation
Publisher
Graduate School of Ewha Womans University
Degree
Master
Abstract
The aim of our current research is to isolate and purify the antifungal compounds produced by Streptomyces willlmorei EMP22 *isolated from soil and to carry out the analysis of their structures in order to develop antifungal antibiotics functioning as a cell wall synthesis-inhibitor without influencing in human body. Antibiotic- producing strain, EMP22 was identified as S. willlmorei(Park, 1995). Antifungal compounds were isolated from the culture filtrate of the strain by use of Amberlite XAD-2(macroreticular resin) column chromatography, Preparative TLC, Sephadex LH-20(gel filtration) column chromatography, and Preparative HPLC. The main principle of the several compounds showing antifungal activities was fractionated and its structure was determined by instrumental analysis such as UV, NMR, and MS techniques. The compound was identified to be tunicamycin Ⅶ, a carcinogenic, antiviral, and antifungal antibiotic produced by S. lysosuperificus. However, this is the first report that tunicamycin Ⅶ has an antifungal activity against Cryptococcus laurentii NCYC 139.;이번 연구의 목적은 인체에는 해를 주지 않으면서 진균의 세포벽 합성을 저해하는 항진균성 항생물질을 개발하는 것이었다. 먼저, 토양에서 분리하여 동정한 Streptomyces willmorei EMP22가 생산하는 항진균성 항생 물질을 분리정제하고, 그것의 구조분석을 시행하였다. 항진균성 물질들은 배양 상등액으로부터 Amberlite XAD-2(macroreticular resin) column chromatography, 분취용 TLC, Sephadex LH-20(gel filtration) chromatography, 그리고 분취용 HPLC를 이용하여 분리되었다. 항진균 활성을 보이는 여러 peaks들 가운데 주요한 하나의 peak을 골라 그것의 fraction을 받아서, UV, NMR, MS techniques을 이용한 구조분석을 수행하였다. 그 결과, 이 물질은 발암물질이며, 항바이러스제이며, 항진균물질인 Tunicamycin Ⅶ 과 구조가 동일하였다. 이 항진균물질은 S. lysosuperificus에 의해 생산되는 것으로 알려져왔다(Tamura, 1982). 그러나 Tunicamycin이 Cryptococcus laurentii NCYC 139 에 대한 항진균 활성을 갖는다는 연구결과는 본 논문이 처음 보고하는 것이다. 본 연구는 C. laurentii 이외에 Aspergillus flavus, A. Penicillium citrinum 에 대한 활성도 조사하였다.
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