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알칼리성 세균 NO-32에 의한 Ethylene 생성과 발효조건 연구

알칼리성 세균 NO-32에 의한 Ethylene 생성과 발효조건 연구
Other Titles
(The) Investigation of Fermentation Condition for Ethylene Production of Alkaiophilic microorganism No-32
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대학원 생물과학과
Ethylene 생성알칼리성 세균 NO-32발효조건
이화여자대학교 대학원
토양으로 부터 분리, 동정된 Micrococcus sp. No-32는 pH 10.3에서 잘 증식하고 ethylene을 생성하는 균주이다. 이 균주로 부터 ethylene 생산성을 높이기 위해 intact cell에 의한 발효조건을 조사하였다. 회분발효시 최적 ethylene 생성온도는 30℃, 초기 pH는 10.2, 초기 접종농도는 0.4 mg of DCW/ml of culture였다. 각종 아미노산, 유기산, 당류의 다양한 기질전환효과를 검토한 결과 대체로 아미노산과 유기산의 효과가 높았다. 특히 methionine에서 효과가 있었으며 적정농도는 균의 증식과 ethylene생성에도 좋은 100 mM로 결정하였다. 빛과 산소에 의한 영향을 살펴보면 빛과 산소가 있을 때가 없을시보다 약 2배에 가까운 ethylene을 생성하였고, 진탕배양이 정치배양보다 5배 높은 ethylene 생성량을 나타냈다. 반연속식 발효시 배양액의 50, 40, 30, 20%를 새 배지로 치환해 주었는데 치환율이 낮을 수록 ethylene 생성속도가 빨라져서 20%치환시 가장 높은 ethylene 생성속도를 나타내었다. 연속식 발효시 희석속도는 0.075h^(-1)일때, 도입 기질농도 100 mM, 도입 배지 농도 5배일때 가장 높은 ethylene 생성속도를 나타내었다. 이상에서 살펴본 바와 같이 Micrococcus sp. No-32는 연속배양을 통한 ethylene 대량생산의 가능성을 보여주었다. 아직 미생물에 의한 ethylene 생성 기작이 정확히 밝혀지지 않았는데, Micrococcus sp. No-32에 의한 ethylene 생성경로와 효소 체계, 미생물내에서의 ethylene의 역할 등의 연구가 이루어져야 할 것이다.;Alkalophilic microorganism No-32, isolated from soil, produced ethylene. The characteristics of this microorgnism resembled micrococcus varians. But this strain was differed from it in several physiological characteristics. The purpose of this work is to optimize the fermentation condition for ethylene production of the strain No-32. Optimal condition for ethylene production was acheived at 30℃, pH 10.2. Inoculum size was 0.4 mg of DCW/ml of culture. Ethylene was effectively produced from 100mM L-methionine. In addition to, this strain was required air, light and agitation for ethylene production. In batch fermentation, ethylene evolution was closely related with cell growth. The most amount of ethylene occured during the exponential phase to stationary hase of growth. But pH and amount of methionine in culture medium were decreased in this period. In semicontinuous fermentation, the interval of replacement was shorted, as the replacement rate was lowed. So ethylene production rate was increased at 20% replacement feeding rate. This result indicated that ethylene production by continuous fermentation is possible. In continuous fermentation, maximum ethylene production rate was observed when dilution rate was 0.075h^(-7), methioinine feeding concentration was 100 mM, medium feeding concentration was 5 fold.
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