Xylazine의 새로운 대사산물의 생체내에서의 동태에 관한 연구

Abstract

xylazine의 대사산물을 확인 및 정량하는 연구를 수행하였다. 추정 대사산물인2,6-dimethylphenylisothiocyanate, 2,6-dimethyl-4-hydroxyaniline , 2,6-dimethylacetanilide, 2,6-dimethyl-4-hydroxyacetanilide, (2,6-dimethyl-4-hydroxyphenyl)isothiocyanate, p-hydroxy-xylazine을 합성하였다. 이 중 Parent drug과 2,6-dimethylphenylisothiocyanate, 2,6dimethyl-4-hydroxyaniline과 p-hydroxy-xylazine을 쥐의 뇨 중에서 GC/MSD로 확인하였다. 이 중에서 주요 대사산물인 p-hydroxy-xylazine과 독성이 있을 것으로 사료되는 2,6-dimethylphenylisothiocyanate와 parent drug 및 2,6-dimethylaniline을 혈액, 뇨, 뇌, 신장, 간, 근육 중에서 GC/NPD로 정량하였다.;Xylazine hydrochloride(Rompun) is a tranquilizer, effective in numerous domestic and wild animals. Because of the intense metabolism and fast excretion of xylazine, it is difficult to identify the parent drug in animals. So, in order to identify the metabolites of xylazine, five possible metabolites of xylazine were synthesized. 2,6-dimethylphenylisothiocyanate, 2,6-dimethyl 4-hydroxyaniline, p-hydroxy-xylazine were identified as metabolites of xylazinein rat urine sample by GC/MSD. As a result of quantitation study, it is concluded that main metabolite of xylazine was p-hydroxy-xylazine and excreted even in 48hr urine sample. The hydroxylation of xylazine was occured very rapidly in liver and this reaction was continued even in 48hr liver sample. Toxic metabolite, 2,6-dimethylphenylisothiocyanate, was found in kidney, liver, and muscle samples.