View : 16 Download: 0

當歸成分이 肝의 藥物代謝 酵素活性에 미치는 影響

Title
當歸成分이 肝의 藥物代謝 酵素活性에 미치는 影響
Other Titles
Effects of the constituent of the roots of Angelica gigas on Hepatic Drug Metabolizing Enzyme Activities
Authors
韓正美
Issue Date
1989
Department/Major
대학원 약학과
Keywords
당귀약물대사효소
Publisher
이화여자대학교 대학원
Degree
Master
Advisors
이인란
Abstract
간의 약물대사효소 활성에 현저한 영향을 미치는 것으로 豫知된 Angelica속 식물 생약중 當歸로 부터 有效成분을 糾明하고자 당귀 MeoH추출물의 係統的 分劃을 통해 얻은 각 분획물중 가장 활성이 큰 ether분획물로 부터 pyranocoumarin계열 물질인 decursin을 분리하였다. 간 약물대사에 미치는 영향에 대해서는 hexobarbital 수면시간, 혈중 hexobarbital 농도측정, aminopyrine, aniline, hexobarbital 대사량 측정 및 Cyt.p-450의 정량등을 지표로 검토한 결과 decursin을 hexobarbital 수면시간 연장, 혈중 hexobarbital 농도증가, 각종 효소활성의 감소, Cyt. P-450의 감소등을 나타내어 간의 약물대사효소 활성 억제작용이 있음이 입증되었다.;The methanolic extract of the roots of Angelica gigas exhibited a significant prolongation of hexobarbital (HB)-induced sleeping time. Systematic fractionation and liquid chromatography of an ether soluble fraction, monitoring by bio-assay, led to the isolation of an active principle which was identified as decursin, a pyranocoumarin by spectral analysis. Decursin, with a single treatment in mice was found to show not only a significant prolongation of HB-induced sleeping time but also an increase in serum HB concentration. It was also noted that decursin caused a significant inhibition of hepatic drug metabolizing enzymes such as aminopyrine N-demethylase , aniline hydroxylase and hexobarbital hydroxylase accompanied by a concomitant loss of cytochrome P-450 concentration. These findings and the results that the destruction of cytochrome P-450 by decursin in vitro required NADPH-generating system strongly suggested that cytochrome P-450 was specifically destroyed in a metabolic process and as a consequence, a significant inhibition of substrate metabolism might be occured.
Fulltext
Show the fulltext
Appears in Collections:
일반대학원 > 생명·약학부 > Theses_Master
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE