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Differential accumulation of transcripts for ACC synthase homologs in etiolated mung bean hypocotyls in response to Li+

Title
Differential accumulation of transcripts for ACC synthase homologs in etiolated mung bean hypocotyls in response to Li+
Authors
이보림
Issue Date
1999
Department/Major
대학원 생물과학과
Publisher
이화여자대학교 대학원
Degree
Master
Abstract
녹두에서 ACC synthase 는 적어도 7개의 유전자 군에 의해 암호화되고,이 유전자 군의 호르몬이나 단백질 합성 저해, 발달 신호, 스트레스 등 다양한자극에 의해 차별적으로 유도되어진다고 알려졌다. Inositol-phophate-phosphatase의 활성화를 억제함으로써 Pl second messenger system을 방해 한다고 알려진 Li^+ 이온은 식물에서 ACC synthase를 활성화하는 강력한 유도체 중 하나이다. 녹두의 하배축 절편에 LiCl를 처리한 후, PT-PCR assay를 통해서 시간별, 농도 증가에 따른 ACC synthase 유전자군의 전사량 변화를 살펴보았다. Li^+의 농도에 비례해서 ACS7 전사체의 발현이 가장 크게 증가 되었다. 특이한 점은 오옥신에 의해서 증가된다고 보고되어진 ACS 유전자들이 Li^+에 의해서도 유도되어짐이 밝혀져 IAA 신호 전달 경로의 Li^+의 관련여부 가능성이 보여진다. 또한 Ca^2+을 처리했을 시에는 처리한 농도 증가에 따라서 Li^+에 의한 전사체 발현이 감소함을 보여서, ACC synthase와 Pl cyclepathway의 연관 가능성을 시사하고 있다. ; In mung bean (Vigna radiate), 1-aminocyclopropane-1-carboxylate syntheses (ACS) is encoded by a multigene family consisting of at least seven members whose expression is induced by hormones, developmental signal, protein synthesis inhibition, and stresses. Li^+, known to interfere with the phosphoinositide (PI) second messenger system by inhibiting the activity of inositol-phophate-phosphatases, is one of the strongest inducers of ACC syntheses activity in plant. Treatment of etiolated mung bean hypocotyls with LiCl results in a rapid induction of the ACS7 of the multigene. The effects of ACS7 are specific, does-dependent. It is reported IAA also induces ACS7. The banes induced by IAA in plant are also induced by Li^+ and this shows there is a possible role of Li^+ in IAA signal transudation pathway. And the effect of Li^+ on the expression can be reversed by Ca^2+. This suggested that the regulation of some ACS gene may involve PI cycle pathway. Since plant contain no Li^+, the action appears to unmask pre-existing biochemical capacity that may be utilized by various unknown transducer during plant growth and development.
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