Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이경은 | - |
dc.creator | 이경은 | - |
dc.date.accessioned | 2016-08-25T06:08:21Z | - |
dc.date.available | 2016-08-25T06:08:21Z | - |
dc.date.issued | 1977 | - |
dc.identifier.other | OAK-000000032912 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/183301 | - |
dc.identifier.uri | http://dcollection.ewha.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000032912 | - |
dc.description.abstract | Leuconostoc mesenteroides No. 20-2-10 isolated from Kim-chi demonstrated to be a good strain for the production of glucose 6-phosphate dehydrogenase, an intracellular enzyme(average 1 u/ml broth). It was demonstrated that the maximum enzyme activity was obtained in the AC medium containing 1.2% sucrose as a carbon source and 1.0% yeast extract and 1.0% tryptone as nitrogen sources. The optimum temperature and pH for the culture of the organism were 30℃ and pH 7.0, respectively. The maximum growth of the cell and the productivity of the enzyme was attained within 10 hours at the condition described above.;김치로 부터 분리된 Leuconostoc mesenteroies No. 20-2-10은 보고된 동종의 균주보다 glucose-6-phosphate 탈수소 효소의 활성이 높은 우수균주임이 판명 되었기에 이 균주의 최적 배양조건을 규명하였다. 이 균주는 탄소원으로 1.2%서당, 질소원으로 1.0% 효모추출물과 1.0% tryptone을 함유하는 AC배지에서 glucose-6-phosphate 탈수소 효소의 생산량이 가장 높았다. 균주 배양의 최적 온도와 PH는 각각 30℃와 7.0 이였다. 이와같은 조건하에서 균주의 최대 증식 및 효소의 최대 생산치는 10시간내에 도달 했음을 관찰하였다. | - |
dc.description.tableofcontents | Ⅰ. INTRODUCTION = 1 Ⅱ. EXPERIMENTAL PROCEDURES = 5 A. Isolation of Leuconostoc strain = 5 B. Cultivation = 6 C. Methods of enzyme assay = 7 D. Materials = 8 Ⅲ. Results and Discussions = 9 A. Selection of enzyme producing strains = 9 B. Identification of the strain = 11 C. Effect of carbon source = 14 D. Effect of nitrogen source = 16 E. Effect of pH = 17 F. Effect of temperature = 17 G. Effect of metal ions and vitamines = 21 H. Time course of cultivation = 21 Ⅳ. CONCLUSION = 25 BIBLIOGRAPHY = 26 | - |
dc.format | application/pdf | - |
dc.format.extent | 2468673 bytes | - |
dc.language | eng | - |
dc.publisher | The Graduate School of Ewha Womans University | - |
dc.subject | 김치 | - |
dc.subject | 균주 | - |
dc.subject | 탄소원 | - |
dc.title | STUDIES OF MICROBIAL GLUCOSE -6- PHOSPHATE DEHYDROGENASE | - |
dc.type | Master's Thesis | - |
dc.format.page | 28 p. | - |
dc.identifier.thesisdegree | Master | - |
dc.identifier.major | 대학원 약학과 | - |
dc.date.awarded | 1977. 8 | - |