Pectin-Gelatin Complex Coacervation에 의한 Indomethacin 의 Microencapsulation에 관한 연구

Abstract

Pectin-gelatin complex coacervation system을 이용하여, 위장관장애가 보고되는 비스테로이드성 소염진통제인 Indomethacin을 핵물질로 하는 microcapsule을 조제하였다. Microencapsulation시의 최적의 pH는 3.8 이었으며, 가장 적절한 pectin과 gelatin의 비율은 1 : 2였다. Pectin용액 및 gelatin용액의 colloid농도를 1%, 1.5% 및 2%로 하였을 때, 농도가 증가할수록 microcapsule의 막두께는 두꺼워졌다 용출속도는 Indomethacin분말에 비해 크게 촉진되었는데 1.5% 및 2%의 colloid의 용액으로 조제한 경우에서는 거의 비슷하였으나, 1% colloid용액으로 조제한 Indomethacin-pectin-gelatin microcapsule보다는 용출이 지연되었다. 즉 pH 7.2인산염완충액 : 증류수( 1 : 4V/V)의 용출용매에서 T_(50%)은 Indomethacin분말이 50분인데 비하여 colloid 농도가 1%, 1.5% 및 2%의 경우는 3분, 5분, 6분으로 더 빨라졌다. Scanntng Electron micrography로 관찰된 Indomethacin-pectingelatin microcapsule은 표면에 주름 (folding과 invagination)이 관찰되었다.;Indomethacin, a non-steroidal antiinflammatory drug inducing gastric irritation, was microencapsulated using pectin-gelatin complex coacervation method. Optimum conditions for microencapsulation and dissolution characteristics of microcapsules were studied. Optimum pH and pectin-gelatin ratio for microencapsulation were 3.8 and 1:2 respectively. As concentration of colloid solution increased, wall thickness of microcapsules were increased. The dissolution rate of Indomethacin-pectin-gelatin microcapsules prepared by 1.5% and 2% colloid solution were similar but slower than that of Indomethacin-pectin-gelatin microcapsules prepared by 1% colloid solution. The 50% release time (T_(50%)) of Indomethacin-pectin-gelatin microcapsules prepared by 1%, 1.5% and 2% colloid solutions were 3min, 5min, and 6min respectively while that of Indomethacin powder was 50min. Surface of microcapsules was found to be folded and invaginated by Scanning Electron Micrography.