View : 23 Download: 0

식용 AZO색소 hepatic microsomal mixed function oxidase system 미치는 영향

Title
식용 AZO색소 hepatic microsomal mixed function oxidase system 미치는 영향
Other Titles
(The) Effects of Food Azo Dyes on the Hepatic Microsomal Mixed Function Oxidase System in Rats
Authors
김백화
Issue Date
1987
Department/Major
대학원 약학과
Keywords
식용AZO색소oxidase system
Publisher
이화여자대학교 대학원
Degree
Master
Abstract
현재 우리나라에서 허용되어 있는 식품 첨가물중 azo색소는 amaranth, tartrazine, sunset yellow 및 allura red로서 모두 분자중에 술폰산기(-SO_(3)H) 및 수산기(-OH)를 가지고 있다. 이러한 수용성의 산성 azo색소가 간 microsome내에서 많은 물질의 활성화와 해독작용을 나타내는 주요 대사과정중 전자전달계 효소인 mixed function oxidase system에 미치는 영향을 알아보고자 각 azo색소들을 80mg/kg으로 흰 쥐의 복강 내에 1일 1회 4일간 투여하고 간 microsome을 분리하여 cytochrome P-450, cytochrome b_(5), NADPH cyt. c-reductase 및 azo reductase의 활성을 측정하였다. 또한 효소 상호간의 의존성을 좀 더 구체적으로 구명하고자 간의 물질대사 효소를 유도시키는 것으로 알려진 phenobarbital 및 3-methylcholanthrene과 물질대사 효소의 합성을 저해하는 것으로 알려진 cobaltous chloride 및 carbon tetrachloride를 각각 투여하고 cytochrome P-450 및 NADPH cyt. creductase 활성에 따른 azo reductase의 활성을 측정하여 다음과 같은 결과를 얻었다. 1. Sulphonate azo 색소는 hepatic microsomal Cyt. P-450 및 Cyt. b_(5)의 함량을 감소시켰다. 2. Sulphonate azo 색소는 hepatic microsomal azo reductase의 활성을 감소시켰으며, 감소된 양상은 Cyt. P-450함량의 감소양상과 동일하였다. 3. Sulphonate azo색소는 NADPH cyt. c-reductase 활성에 영향을 미치지 않았다. 4. Azo reductase활성은 3-methylcholanthrene 투여에 의해서도 증가되었다.;The four azo dyes such as Amaranth (FD & C Red No.2), Tartrazine (FD & C Yellow No.4), sunset Yellow (FD & C Yellow No.5) and Allura red (FD & C Red No.40) are currently employed as a food additives in Korea. In this study, the effects of these azo dyes on the hepatic microsomal mixed function oxidase systems in Rats. (i.e., Cyt. P-450, Cyt. b_(5), NADPH cyt. c-reductase and azo reductase) were investigated. Furthermore, to determine the relationship among the electron transport systems, each level of azo reductase, Cyt. p-450 and NADPH cyt. c-reductase were measured upon the administration of phenobarbital (known as an inducer of Cyt. p-450), 3-methylcholanthrene (Known as an inducer of Cyt. p-448), CoCℓ_(2) (inhibitor on Cyt. p-450) or (CCℓ_(4) (inhibitor on Cyt. p-450). The results of these studies are as follows; 1. The level of Cyt. p-450 and Cyt. b_(5) were decreased upon the administration of these azo dyes. 2. When the level of Cyt. p-450 was decreased, the azo reductase activity was also decreased. 3. These azo dyes did not show any significant effect on the level of NADPH cyt. c-reductase. 4. The administration of 3-methylcholanthrene resulted in the elevation of azo reductase activity. The 3-methylcholanthrene may be responsible for the induction of CO-insensitive electron transport system.
Fulltext
Show the fulltext
Appears in Collections:
일반대학원 > 생명·약학부 > Theses_Master
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE