Electrospinnig of collagen typeⅠ nanofibers on anodic oxidized Titanium surface enhancing osteoblast-like cell attachment

Abstract

타이타늄은 그 생체친화적인 특성과 물성적인 장점 때문에 치과에서 임플란트 재료료 많이 쓰이는 금속이다. 임플란트의 이식 효과를 최대화 하기 위해 금속의 표면개질이 많이 행해지고 있으며, 이번 실험에서는 전기방사방법을 이용하여 Ti-금속으로 구성되고 산화피막처리된 치아용 임플란트의 표면에 콜라겐 나노섬유를 코팅하는 방법을 고안하였다. 콜라겐을 가교하여 나노섬유의 모양이 훼손되는 것을 방지하였으며, 임플란트의 표면에 조골세포의 초기 부착실험을 통하여 제조된 임플란트가 생체친화성을 증가시켰음을 알 수 있었다. 이는 전기분사방법을 통한 코팅방법이 치주용 임플란트의 코팅에 적합함을 확인하게 해준다.;Titanium is the metals of choice in dental applications because of good mechanical properties. To maximize the effect of dental implant, Surface modification of Ti with anodic oxidation and electrospinning of collagen nanofiber was suggested. Titanium discs were prepared anodic oxidized treatment(anodized disc) and nontreatmnet(machined disc). Collagen was dissolved in 1,1,1,3,3,3, Hexafluoro-2-Propanol(HFP) at 8%(w/v) and electrospun to disc at different times. After electrospinning, collagen nanofiber coated Ti discs were cross-linked by treatment with 25% glutaraldehyde vapor. Collagen quantification test and stability test were followed. And human osteoblast-like cells (HOS) were used for cell attachment assays. Electrospinning of collagen using HFP as a solvent get continuous collagen nanofibers that mean diameter is 200nm. After crosslinking treatment, mean diameter and roughness was slightly increased. Collagen loading doses are 0.2mg to 0.8mg. Collagen nanofiber coated Ti implant surface potentially improved initial cell attachment. Collagen coating with electrospinning method might be an effective tool to provide cell compatible surface.