Full metadata record
DC Field | Value | Language |
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dc.contributor.advisor | 서은경 | - |
dc.contributor.author | 김향림 | - |
dc.creator | 김향림 | - |
dc.date.accessioned | 2016-08-25T04:08:53Z | - |
dc.date.available | 2016-08-25T04:08:53Z | - |
dc.date.issued | 2004 | - |
dc.identifier.other | OAK-000000009805 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/178265 | - |
dc.identifier.uri | http://dcollection.ewha.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000009805 | - |
dc.description.abstract | Activity-guided fractionation of the EtOAc-soluble fraction of the MeOH extract of C. pallida using a bioassay based on the inhibition of lipopolysaccharide (LPS)-induced PGE₂ production (IC_(50) = 3.6 μg/mL) in murine macrophage RAW 264.7 cells led to the isolation and identification of two amides, N-trans-feruloylmethoxytyramine (1) and N-trans-feruloyltyramine (2) along with seven protoberberine alkaloid derivatives, ophiocarpine (3), berberine (4), 8-oxocoptisine (5), (-)-corydalmine (7), tetrahydropalmatine (8), yuanhunine (9), corydalidzine (10) and corybulbine (11) and one benzophenanthridine type alkaloid, 8-acetonyldihydrosanguinarine (6). N-trans-feruloylmethoxytyramine (1), N-trans-feruloyltyramine (2), and corydalidzine (10) exhibited potent inhibitory activities on lipopolysaccharides (LPS)-induced PGE2 production in murine macrophage RAW 264.7 cells with the inhibitions of 92.61, 68.14, and 95.09 %, respectively. However, compounds 3-9 and 11 were inactive in this assay system. The structures of 1-11 were determined by analysis of physical and spectroscopic data (mp, UV, IR, MS, ¹H-, ^(13)C-, HSQC, HMBC and COSY NMR) as well as by comparison with published values. Isolation, structural identification and the biological evaluation of the compounds are described herein.;Corydalis pallida (Thunb.) Pers. 괴불주머니 (양귀비과)의 CHCl₃분획 추출물은 COX-2에 의한 PGE₂ 생성을 효과적으로 저해하는 활성을 보였다. 이러한 활성에 따른 분획을 실시하여, 물질을 분리하였다. 물질의 분리를 위해서 open column chromatography, sephadex, reverse column(YMC GEL ODS-A) chromatography등을 이용하였고, spectroscopic data (mp, MS, ¹H-, ^(13)C-, HSQC, HMBC and COSY NMR)와 문헌 조사를 통해 분리된 물질의 구조를 분석, 결정하였다. 그 결과, 2가지 주요 활성 물질, N-trans-feruloylmethoxytyramine (1) (% inhibition = 92.61)과 corydalidzine (10) (% inhibition = 95.09)을 분리하였다. N-trans-feruloyltyramine (2) (% inhibition = 68.14)는 약한 COX-2 효소 저해 활성을 보인 반면, 추가적으로 분리된, 9개의 물질, ophiocarpine (3), berberine (4), 8-oxocoptisine (5), 8-acetonyldihydrosanguinarine (6), (-)-corydalmine (7), tetrahydropalmatine (8), yuanhunine (9), corybulbine (11)은 50% 이하의 inhibition 값을 보여 활성이 없는 것으로 판단되었다. | - |
dc.description.tableofcontents | 목차 Ⅰ. INTRODUCTION and SCOPE OF STUDY = 13 A. Previous studies on Corydalis pallida = 14 B. Cyclooxygenase inhibitory activity = 14 C. Scope of study = 15 Ⅱ.EXPERIMENTAL = 17 A. Plant material = 18 B. General experimental procedures = 18 C. Solvent extraction and partitioning of C. pallida = 19 D. Measurements of PGE2 accumulation by COX-2 in cultured LPS-induced RAW 264.7 cells = 20 E. Column chromatography of the EtOAc extracts = 21 1. Isolation and characterization of N-trans-feruloylmethoxytyramine (1) = 23 2. Isolation and characterization of N-trans-feruloyltyramine (2) = 24 3. Isolation and characterization of ophiocarpine (3) = 25 4. Isolation and characterization of berberine (4) = 26 5. Isolation and characterization of 8-oxocoptisine (5) = 27 6. Isolation and characterization of 8-acetonyldihydrosanguinarine (6) = 28 7. Isolation and characterization of (-)-corydalmine (7), tetrahydropalmatine (8), yuanhunine (9), corydalidzine (10) and corybulbine (11) = 29 F. COX inhibitory activity = 34 Ⅲ. DISCUSSION = 35 A. Characterization of the isolates from C. pallida = 36 1. Identification of N-trans-feruloylmethoxytyramine (1) = 36 2. Identification of N-trans-feruloyltyramine (2) = 36 3. Identification of ophiocarpine (3) = 37 4. Identification of berberine (4) = 46 5. Identification of 8-oxocoptisine (5) = 47 6. Identification of 8-acetonyldihydrosanguinarine (6) = 48 7. Identification of (-)-corydalmine (7), tetrahydropalmatine (8), yuanhunine (9), corydalidzine (10) and corybulbine (11) = 50 B. COX-2 inhibitory activity evaluation = 55 C.Conclusion = 55 APPENDIX = 57 REFERENCES = 60 논문 초록 = 64 | - |
dc.format | application/pdf | - |
dc.format.extent | 672066 bytes | - |
dc.language | eng | - |
dc.publisher | 이화여자대학교 약학대학원 | - |
dc.title | Isolation and Structure Identification of Bioactive Constituents of Corydalis pallida | - |
dc.type | Master's Thesis | - |
dc.format.page | 1 책 | - |
dc.identifier.thesisdegree | Master | - |
dc.identifier.major | 대학원 약학과 | - |
dc.date.awarded | 2005. 2 | - |