DSpace Community:
https://dspace.ewha.ac.kr/handle/2015.oak/171823
2024-03-29T09:41:42Z
2024-03-29T09:41:42Z
Identification of dendritic cell precursor from the CD11c<SUP>+</SUP> cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand
박채규
https://dspace.ewha.ac.kr/handle/2015.oak/267127
2024-02-08T16:30:59Z
2023-01-01T00:00:00Z
Title: Identification of dendritic cell precursor from the CD11c<SUP>+</SUP> cells expressing high levels of MHC class II molecules in the culture of bone marrow with FLT3 ligand
Ewha Authors: 박채규
Abstract: Dendritic cells (DCs) are readily generated from the culture of mouse bone marrow (BM) treated with either granulocyte macrophage-colony stimulating factor (GM-CSF) or FMS-like tyrosine kinase 3 ligand (FLT3L). CD11c(+)MHCII(+) or CD11c(+)MHCII(hi) cells are routinely isolated from those BM cultures and generally used as in vitro-generated DCs for a variety of experiments and therapies. Here, we examined CD11c(+) cells in the BM culture with GM-CSF or FLT3L by staining with a monoclonal antibody 2A1 that is known to recognize mature or activated DCs. Most of the cells within the CD11c(+)MHCII(hi) DC gate were 2A1(+) in the BM culture with GM-CSF (GM-BM culture). In the BM culture with FLT3L (FL-BM culture), almost of all the CD11c(+)MHCII(hi) cells were within the classical DC2 (cDC2) gate. The analysis of FL-BM culture revealed that a majority of cDC2-gated CD11c(+)MHCII(hi) cells exhibited a 2A1(-)CD83(-)CD115(+)CX(3)CR1(+) phenotype, and the others consisted of 2A1(+)CD83(+)CD115(-)CX(3)CR1(- )and 2A1(-)CD83(-)CD115(-)CX(3)CR1(-) cells. According to the antigen uptake and presentation, morphologies, and gene expression profiles, 2A1(-)CD83(-)CD115(-)CX(3)CR1(-) cells were immature cDC2s and 2A1(+)CD83(+)CD115(-)CX(3)CR1(-) cells were mature cDC2s. Unexpectedly, however, 2A1(-)CD83(-)CD115(+)CX(3)CR1(+) cells, the most abundant cDC2-gated MHCII(hi )cell subset in FL-BM culture, were non-DCs. Adoptive cell transfer experiments in the FL-BM culture confirmed that the cDC2-gated MHCIIhi non-DCs were precursors to cDC2s, i.e., MHCIIhi pre-cDC2s. MHCIIhi pre-cDC2s also expressed the higher level of DC-specific transcription factor Zbtb46 as similarly as immature cDC2s. Besides, MHCIIhi pre-cDC2s were generated only from pre-cDCs and common DC progenitor (CDP) cells but not from monocytes and common monocyte progenitor (cMoP) cells, verifying that MHCII(hi )pre-cDC2s are close lineage to cDCs. All in all, our study identified and characterized a new cDC precursor, exhibiting a CD11c(+)MHCII(hi)CD115(+)CX(3)CR1(+) phenotype, in FL-BM culture.
2023-01-01T00:00:00Z
Altered interictal serum histamine and immunoglobulin E but unchanged tryptase levels in individuals with episodic and chronic migraine
박채규
https://dspace.ewha.ac.kr/handle/2015.oak/265169
2023-12-18T16:31:02Z
2023-01-01T00:00:00Z
Title: Altered interictal serum histamine and immunoglobulin E but unchanged tryptase levels in individuals with episodic and chronic migraine
Ewha Authors: 박채규
Abstract: BackgroundSerum histamine, immunoglobulin E, and tryptase are markers of allergic diseases. Despite the reported association between migraine and allergic diseases, differences in these marker levels between episodic and chronic migraines remain unelucidated. MethodsWe investigated serum histamine, immunoglobulin E, and tryptase levels in 97 and 96 participants with episodic migraine and chronic migraine, respectively, and 56 controls according to the presence of allergic diseases. ResultsSerum histamine levels in episodic migraine (median and interquartile ranges, 0.78 [0.65-1.25] ng/mL, p < 0.001) and chronic migraine (0.89 [0.67-1.28] ng/mL, p < 0.001) participants were significantly lower than those in healthy controls (1.19 [0.81-2.08] ng/mL) among the 160 participants without allergic diseases. Serum immunoglobulin E levels in episodic migraine and chronic migraine participants with allergic diseases negatively correlated with headache frequency (correlation coefficient = -0.263, p = 0.017). Serum histamine levels in participants with allergic diseases and serum immunoglobulin E levels in participants without allergic diseases were not significantly different among episodic migraine, chronic migraine, and control groups. Serum tryptase levels did not significantly differ among episodic migraine, chronic migraine, and control participants with and without allergic diseases. ConclusionsAltered serum histamine and immunoglobulin E levels in episodic migraine and chronic migraine and different profiles concerning allergic diseases suggest the involvement of allergic mechanisms in migraine pathogenesis.
2023-01-01T00:00:00Z
No change in interictal C-reactive protein levels in individuals with episodic and chronic migraine: A case-control study and literature review
박채규
https://dspace.ewha.ac.kr/handle/2015.oak/262996
2023-12-18T16:31:09Z
2022-01-01T00:00:00Z
Title: No change in interictal C-reactive protein levels in individuals with episodic and chronic migraine: A case-control study and literature review
Ewha Authors: 박채규
Abstract: ObjectivesThe levels of some migraine biomarkers differ between episodic migraine (EM) and chronic migraine (CM), but information on C-reactive protein (CRP) levels in EM and CM is conflicting. Thus, this study aimed to evaluate CRP levels in participants with EM and CM in comparison to those in healthy controls. MethodsPlasma CRP levels were evaluated by high-sensitivity CRP tests in female participants with EM (n = 174) and CM (n = 191) and healthy controls (n = 50). ResultsThe results showed no significant difference in CRP levels among the EM, CM, and control groups (median and interquartile range, 0.40 [0.15-0.70] mg/L vs. 0.40 [0.15-1.00] mg/L vs. 0.15 [0.15-0.90] mg/L, p = 0.991). The ratio of individuals with elevated CRP levels (>3.0 mg/L) did not significantly differ among the EM, CM, and control groups (3.4% [6/174] vs. 2.1% [4/191] vs. 0.0% [0/50], p = 0.876). Multivariable regression analyses revealed that CRP levels were not significantly associated with headache frequency per month (beta = -0.076, p = 0.238), the severity of anxiety (Generalized Anxiety Disorder-7 score, beta = 0.143, p = 0.886), and depression (Patient Health Questionnaire-9 score, beta = 0.143, p = 0.886). Further, CRP levels did not significantly differ according to clinical characteristics, fibromyalgia, medication overuse, preventive treatment, and classes of preventive treatment medications. Among participants with a body mass index >= 25 kg/m(2), the CRP levels in EM (n = 41) and CM (n = 17) were numerically higher than those in the control (n = 6) (1.30 [0.28-4.25] mg/L vs. 1.10 [0.50-3.15] mg/L vs. 0.40 [0.15-0.83] mg/L, p = 0.249) but did not reach statistical significance. ConclusionsThe interictal CRP level is not likely to be a biomarker for EM or CM.
2022-01-01T00:00:00Z
Foxc1 and foxc2 in the neural crest are required for ocular anterior segment development
서승운
https://dspace.ewha.ac.kr/handle/2015.oak/247434
2024-02-20T16:34:25Z
2017-01-01T00:00:00Z
Title: Foxc1 and foxc2 in the neural crest are required for ocular anterior segment development
Ewha Authors: 서승운
Abstract: PURPOSE. The large Forkhead (Fox) transcription factor family has essential roles in development, and mutations cause a wide range of ocular and nonocular disease. One member, Foxc2 is expressed in neural crest (NC)-derived periocular mesenchymal cells of the developing murine eye; however, its precise role in the development, establishment, and maintenance of the ocular surface has yet to be investigated. METHODS. To specifically delete Foxc2 from NC-derived cells, conditional knockout mice for Foxc2 (NC-Foxc2-/-) were generated by crossing Foxc2F mice with Wnt1-Cre mice. Similarly, we also generated compound NC-specific mutations of Foxc2 and a closely related gene, Foxc1 (NC-Foxc1-/-;NC-Foxc2-/-) in mice. RESULTS. Neural crest-Foxc2-/- mice show abnormal thickness in the peripheral-to-central corneal stroma and limbus and displaced pupils with irregular iris. The neural crest-specific mutation in Foxc2 also leads to ectopic neovascularization in the cornea, as well as impaired ocular epithelial cell identity and corneal conjunctivalization. Compound, NC-specific Foxc1; Foxc2 homozygous mutant mice have more severe defects in structures of the ocular surface, such as the cornea and eyelids, accompanied by significant declines in the expression of another key developmental factor, Pitx2, and its downstream effector Dkk2, which antagonizes canonical Wnt signaling. CONCLUSIONS. The neural crest-Foxc2 mutation is associated with corneal conjunctivalization, ectopic corneal neovascularization, and disrupted ocular epithelial cell identity. Furthermore, Foxc2 and Foxc1 cooperatively function in NC-derived mesenchymal cells to ensure proper morphogenesis of the ocular surface via the regulation of Wnt signaling. Together, Foxc2 is required in the NC lineage for mesenchymal-epithelial interactions in corneal and ocular surface development. © 2017 The Authors.
2017-01-01T00:00:00Z