> ! \pJava Excel API v2.6 Ba==h\:#8X@"1Arial1Arial1Arial1Arial + ) , * `KDC,title[*]contributor[author]contributor[advisor]keywords[*]date[issued] publisher citationsidentifier[uri]identifier[doi]abstractq2D and 3D Hybrid Systems for Enhancement of Chondrogenic Differentiation of Tonsil-derived Mesenchymal Stem CellsѼ85002015tTŐYP Y~http://dspace.ewha.ac.kr/handle/2015.oak/212228;
http://dcollection.ewha.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000111758;
2D/3D hybrid cell culture systems were constructed by increasing temperature to 37 oC of the thermogelling poly(ethylene glycol)-poly(L-alanine) diblock copolymer (PEG-L-PA) aqueous solution containing tonsil-tissue-derived mesenchymal stem cells (TMSCs) and graphene (G) or graphene oxide (GO). When the TMSCs were cultured on the two dimensional (2D) culture systems of G and GO by using the growth medium of high glucose Dulbecco s modified eagle medium (DMEM), the cell exhibited a fibroblast-like morphology with little expression of the differentiation biomarkers such as osteocalcin (OCN), collagen type II (COL II), and peroxisome proliferator-activated receptor gamma (PPAR). On the other hand, the spherical cell morphology were observed in 2D/3D hybrid culture systems of G/PEG-L-PA and GO/PEG-L-PA by using the same growth medium of DMEM. The cell proliferations were 30-50 % higher in G/PEG-L-PA than GO/PEG-L-PA, whereas chondrogenic biomarker of COL II was highly observed in 2D/3D hybrid systems in proportional to the concentration of G or GO. When chondrogenic culture media enriched with TGF-3 (10 ng/mL) was used in the 2D/3D hybrid systems, cells extensively aggregated, and chondrogenic biomarkers of SOX 9, COL II A1, COL II, and COL X were significantly increased. In particular, the COL II A1, COL II, and COL X increased about 10 times by GO/PEG-L-PA hybrid system, compared with PEG-L-PA system.
This paper proved that 1) COL II and TGF-3 synergistically contribute to the chondrogenesis of stem cells, 2) a 2D/3D hybrid culture system provides a very effective in controlling the stem cell differentiation, and 3) the surface properties of incorporated 2D materials play an important role in inducing specific biomarkers from the encapsulated cells.
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